Application of real-time PCR for tree nut allergen detection in processed foods.

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2019
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Abstract
Currently, food allergies are an important health concern worldwide. The presence of undeclared allergenic ingredients or the presence of traces of allergens due to accidental contamination during food processing poses a great health risk to sensitized individuals. Therefore, reliable analytical methods are required to detect and identify allergenic ingredients in food products. Real-time PCR allowed a specific and accurate amplification of allergen sequences. Some processing methods could induce the fragmentation and/or degradation of genomic DNA and some studies have been performed to analyze the effect of processing on the detection of different targets, as thermal treatment, with and without applying pressure. In this review, we give an updated overview of the applications of real-time PCR for the detection of allergens of tree nut in processed food products. The different variables that contribute to the performance of PCR methodology for allergen detection are also review and discussed.
Reference Key
linacero2019applicationcritical Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Linacero, Rosario;Sanchiz, Africa;Ballesteros, Isabel;Cuadrado, Carmen;
Journal critical reviews in food science and nutrition
Year 2019
DOI
10.1080/10408398.2018.1557103
URL
Keywords
real-time pcr allergen detection pressure processing processed foods thermal processing tree nut

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