the marine-derived fungus clonostachys rosea, source of a rare conjugated 4-me-6e,8e-hexadecadienoic acid reducing viability of mcf-7 breast cancer cells and gene expression of lipogenic enzymes

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ID: 245247
2015
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Abstract
A marine-derived strain of Clonostachys rosea isolated from sediments of the river Loire estuary (France) was investigated for its high lipid production. The fungal strain was grown on six different culture media to explore lipid production changes. An original branched conjugated fatty acid, mainly present in triglycerides and mostly produced when grown on DCA (23% of total fatty acid composition). It was identified as 4-Me-6E,8E-hexadecadienoic on the basis of spectroscopic analyses. This fatty acid reduced viability of MCF-7 breast cancer cells in a dose dependent manner (up to 63%) at physiological free fatty acid human plasma concentration (100 μM). Reduction of gene expression of two lipogenic enzymes, the acetyl CoA carboxylase (ACC) and the fatty acid synthase (FAS) was evaluated to explore the mechanisms of action of 4-Me-6E,8E-16:2 acid. At 50 μM, 50% and 35% of mRNA gene expression inhibition were observed for ACC and FAS, respectively.
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dias2015marinethe Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Ana Camila Dos Santos Dias;Nicolas Ruiz;Aurélie Couzinet-Mossion;Samuel Bertrand;Muriel Duflos;Yves-François Pouchus;Gilles Barnathan;Hassan Nazih;Gaetane Wielgosz-Collin
Journal jixie gongcheng xuebao/journal of mechanical engineering
Year 2015
DOI
10.3390/md13084934
URL
http://www.mdpi.com/1660-3397/13/8/4934
Keywords
Cancer conjugated fatty acid MCF-7 fatty acids lipids lipogenesis fatty acid synthasebiology (general)

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