supported membranes meet flat fluidics: monitoring dynamic cell adhesion on pump-free microfluidics chips functionalized with supported membranes displaying mannose domains
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2013
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Abstract
In this paper we demonstrate the combination of supported membranes and so-called flat microfluidics, which enables one to manipulate liquids on flat chip surfaces via “inverse piezoelectric effect”. Here, an alternating external electric field applied to the inter-digital transducers excites a surface acoustic wave on a piezoelectric substrate. Employing lithographic patterning of self-assembled monolayers of alkoxysilanes, we successfully confine a free-standing, hemi-cylindrical channel with the volume of merely 7 µL . The experimentally determined maximum flow velocity scales linearly with the acoustic power, suggesting that our current setup can drive liquids at the speed of up to 7 cm/s (corresponding to a shear rate of 280 s−1) without applying high pressures using a fluidic pump. After the establishment of the functionalization of fluidic chip surfaces with supported membranes, we deposited asymmetric supported membranes displaying well-defined mannose domains and monitored the dynamic adhesion of E. Coli HB101 expressing mannose-binding receptors. Despite of the further technical optimization required for the quantitative analysis, the obtained results demonstrate that the combination of supported membranes and flat fluidics opens a large potential to investigate dynamic adhesion of cells on biofunctional membrane surfaces with the minimum amount of samples, without any fluidic pump.
| Reference Key |
tanaka2013materialssupported
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|---|---|
| Authors | ;Motomu Tanaka;Achim Wixforth;Zeno Guttenberg;Jochen Oelke;Thomas Kaindl;Andreea Pasc |
| Journal | Nature Materials |
| Year | 2013 |
| DOI |
10.3390/ma6020669
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| URL | |
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