cartilage tissue engineering with silk fibroin scaffolds fabricated by indirect additive manufacturing technology

Clicks: 345
ID: 217514
2014
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #87 of 461 articles by views in Nature Materials

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 461 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Advanced tissue engineering (TE) technology based on additive manufacturing (AM) can fabricate scaffolds with a three-dimensional (3D) environment suitable for cartilage regeneration. Specifically, AM technology may allow the incorporation of complex architectural features. The present study involves the fabrication of 3D TE scaffolds by an indirect AM approach using silk fibroin (SF). From scanning electron microscopic observations, the presence of micro-pores and interconnected channels within the scaffold could be verified, resulting in a TE scaffold with both micro- and macro-structural features. The intrinsic properties, such as the chemical structure and thermal characteristics of SF, were preserved after the indirect AM manufacturing process. In vitro cell culture within the SF scaffold using porcine articular chondrocytes showed a steady increase in cell numbers up to Day 14. The specific production (per cell basis) of the cartilage-specific extracellular matrix component (collagen Type II) was enhanced with culture time up to 12 weeks, indicating the re-differentiation of chondrocytes within the scaffold. Subcutaneous implantation of the scaffold-chondrocyte constructs in nude mice also confirmed the formation of ectopic cartilage by histological examination and immunostaining.
基于增材制造（AM）的先进组织工程（TE）技术能够制备具有适于软骨再生的三维（3D）环境的支架。具体而言，AM技术可实现复杂构型特征的引入。本研究采用丝素蛋白（SF），通过间接增材制造方法制备了3D组织工程支架。扫描电子显微镜观察证实，支架内部存在微孔和互连通道，从而赋予了该组织工程支架兼具微观与宏观结构的特征。经间接增材制造工艺后，SF的化学结构和热学特性等固有性质得以保留。利用猪关节软骨细胞在SF支架内进行体外细胞培养，结果显示细胞数量在第14天前呈稳定增长趋势。在长达12周的培养期内，软骨特异性细胞外基质成分（II型胶原）的单位细胞合成量随培养时间延长而增加，表明软骨细胞在支架内发生了再分化。组织学检查和免疫染色结果亦证实，将支架-软骨细胞构建体皮下植入裸鼠体内可诱导异位软骨的形成。
Reference Key
chen2014materialscartilage Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Chih-Hao Chen;Jolene Mei-Jun Liu;Chee-Kai Chua;Siaw-Meng Chou;Victor Bong-Hang Shyu;Jyh-Ping Chen
Journal Nature Materials
Year 2014
DOI
10.3390/ma7032104
URL
http://www.mdpi.com/1996-1944/7/3/2104
Keywords
chondrocytes silk fibroin scaffold electronics civil engineering (general)microscopydescriptive and experimental mechanics nuclear engineeringengineering (general)

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.