A Novel Fluorescent Reporter System Identifies Laminin-511/521 as Potent Regulators of Cardiomyocyte Maturation.

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2020
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Abstract
Pluripotent stem cell-derived cardiomyocytes (PSC-CMs) hold great promise for disease modeling and drug discovery. However, PSC-CMs exhibit immature phenotypes in culture, and the lack of maturity limits their broad applications. While physical and functional analyses are generally used to determine the status of cardiomyocyte maturation, they could be time-consuming and often present challenges in comparing maturation-enhancing strategies. Therefore, there is a demand for a method to assess cardiomyocyte maturation rapidly and reproducibly. In this study, we found that Myomesin-2 (Myom2), encoding M-protein, is upregulated postnatally, and based on this, we targeted TagRFP to the Myom2 locus in mouse embryonic stem cells. Myom2-RFP PSC-CMs exhibited more mature phenotypes than RFP cells in morphology, function and transcriptionally, conductive to sarcomere shortening assays. Using this system, we screened extracellular matrices (ECMs) and identified laminin-511/521 as potent enhancers of cardiomyocyte maturation. Together, we developed and characterized a novel fluorescent reporter system for the assessment of cardiomyocyte maturation and identified potent maturation-enhancing ECMs through this simple and rapid assay. This system is expected to facilitate use of PSC-CMs in a variety of scientific and medical investigations.
Reference Key
chanthra2020ascientific Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Chanthra, Nawin;Abe, Tomoyuki;Miyamoto, Matthew;Sekiguchi, Kiyotoshi;Kwon, Chulan;Hanazono, Yutaka;Uosaki, Hideki;
Journal Scientific reports
Year 2020
DOI
10.1038/s41598-020-61163-3
URL
Keywords
food science antioxidant activity bioaccessibility food analysis agriculture harvest bound and free polyphenol dry season in vitro gastrointestinal digestion maturity cassava (manihot esculenta crantz) stability of antioxidant potential wet season

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