Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017.

Clicks: 260
ID: 96355
2019
Article Quality & Performance Metrics
Overall Quality Improving Quality
0.0 /100
Combines engagement data with AI-assessed academic quality
AI Quality Assessment
Not analyzed
Abstract
Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver derivates are considered the major risk products, and studies focused on the presence of HEV in pig muscles are scarce. The objective of the present study was to evaluate the presence of HEV in different organs and tissues of 45 apparently healthy pigs from nine Spanish slaughterhouses (50% national production) that could enter into the food supply chain. Anti-HEV antibodies were evaluated in serum by an ELISA test. Ten samples from each animal were analyzed for the presence of HEV RNA by reverse transcription real-time PCR (RT-qPCR). The overall seroprevalence obtained was 73.3% (33/45). From the 450 samples analyzed, a total of 26 RT-qPCR positive samples were identified in the liver (7/45), feces (6/45), kidney (5/45), heart (4/45), serum (3/45), and diaphragm (1/45). This is the first report on detection of HEV RNA in kidney and heart samples of naturally infected pigs. HEV RNA detection was negative for rib, bacon, lean ham, and loin samples. These findings indicate that pig meat could be considered as a low risk material for foodborne HEV infection.
Reference Key
garca2019occurrencefrontiers Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors García, Nerea;Hernández, Marta;Gutierrez-Boada, Maialen;Valero, Antonio;Navarro, Alejandro;Muñoz-Chimeno, Milagros;Fernández-Manzano, Alvaro;Escobar, Franco Matías;Martínez, Irene;Bárcena, Carmen;González, Sergio;Avellón, Ana;Eiros, Jose M;Fongaro, Gislaine;Domínguez, Lucas;Goyache, Joaquín;Rodríguez-Lázaro, David;
Journal Frontiers in microbiology
Year 2019
DOI
10.3389/fmicb.2019.02990
URL
Keywords
prevalence food safety seroprevalence pigs slaughterhouse hepatitis e virus pig organs pork cuttings

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.