Rapid detection of porcine DNA in processed food samples using a streamlined DNA extraction method combined with the SYBR Green real-time PCR assay.

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ID: 70241
2020
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Abstract
A specialized DNA extraction method and a SYBR Green quantitative polymerase chain reaction (SyG-qPCR) assay were combined to generate a ready-to-use kit for rapid detection of porcine admixtures in processed meat products. Our qPCR assay utilized repetitive LINE-1 elements specific to the genome of Sus scrofa domesticus (pig) as a target and incorporated internal controls. We improved the genomic DNA extraction method, and reduced extraction times to the minimum. The method was validated for specificity, sensitivity (0.001% w/w) and robustness, and values were compared with those of a commercially available kit. We also tested our method using 121 processed food products and consistently detected amplification only in samples containing pork. Due to its efficiency and cost-effectiveness, our method represents a valuable new method for detecting food adulteration with pork that is superior to existing quality control approaches.
Reference Key
tan2020rapidfood Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Tan, Lee Lee;Ahmed, Siti Aminah;Ng, Siew Kit;Citartan, Marimuthu;Raabe, Carsten A;Rozhdestvensky, Timofey S;Tang, Thean Hock;
Journal Food chemistry
Year 2020
DOI
S0308-8146(19)31780-7
URL
Keywords
adulteration with pork food authentication genomic dna extraction melting curve analysis sybr green pcr sus scrofa

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