Application of a recombinant laccase-chlorogenic acid system in protein crosslink and antioxidant properties of the curd.

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ID: 64240
2018
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Ranked #28 of 67 articles by views in food research international (ottawa, ont)

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Abstract
Milk protein crosslink through the action of enzymes represents a feasible strategy to impart new functionalities to cheese. In this work we reported the effects of a laccase mediator system (LMS) on protein crosslink and antioxidant property of curd. The crosslinking activity of a purified recombinant laccase Ery4 and a commercial enzyme preparation (cLC), with three mediators was firstly evaluated in milk and then applied before curd manufacture. Only Ery4-LMS significantly increased curd weight compared to that of the control sample. SDS-PAGE revealed that similar high molecular weight bands produced by both LMSs in milk were also retained in curds. The antioxidant activity recorded in curds with Ery4-LMS was the highest among all samples both before and after gastro-pancreatic digestion. This is the first time that a CGA-based LMS is used in manufacture of curd with improved antioxidant properties. These results open new perspectives for dairy applications.
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loi2018applicationfood Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Loi, M;Quintieri, L;Fanelli, F;Caputo, L;Mulè, G;
Journal food research international (ottawa, ont)
Year 2018
DOI
S0963-9969(18)30058-9
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