optimizations of α-amylase production by response surface methodology in immobilization bacillus amyloliquefaciens atcc 23350
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ID: 253865
2016
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Abstract
Introduction: Production of an endogenous α-amylase from Bacillus amyloliquefaciens ATCC 23350 was studied and enhanced.
Materials and methods: Protein and carbon sources were analyzed for free and immobilized bacterial cells and number of beads was considered for immobilized cells via one factor at a time methodforα-amylase production by Bacillus amyloliquefaciens. Subsequently, optimization condition was employed solely for immobilized bacterial cells by response surface methodology (RSM).
Results: Peptone and rice starch showed to improve the α-amylase production in immobilized Bacillus cells. RSM generated a mathematical model explaining the optimum concentration of the efficient nutrients (139.35 g/l of rice starch and 80.00 g/l of peptone) leading to an optimum amylase production (205 U/ml).
Discussion and conclusion: The statistical advance displayed significant outcomes to optimize the process parameters for maximal α-amylase production using Bacillus amyloliquefaciens and gave permission to rapid screening of variables. RSM led to find out an immense improvement in enzyme activity (more than 90%: from 25 to 225 U/ml) for the first time.
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samadlouie2016biologicaloptimizations
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| Authors | ;Hamid reza Samadlouie;Maryam Karimiroozbahani;Hami kaboosi;Naser Farrokhi |
| Journal | Epidemiology and psychiatric sciences |
| Year | 2016 |
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