determinação de aflatoxina b1 em rações e aflatoxina m1 no leite de propriedades do estado de são paulo determination of aflatoxin b1 in animal feed and aflatoxin m1 in milk in dairy farms of são paulo state

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ID: 130887
2010
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Abstract
A ocorrência de aflatoxina B1 (AFB1) em rações e aflatoxina M1 (AFM1) no leite cru foi avaliada em propriedades leiteiras situadas na região nordeste do Estado de São Paulo, Brasil, de outubro de 2005 a fevereiro de 2006. A análise de aflatoxinas foi efetuada utilizando-se colunas de imunoafinidade para purificação dos extratos, sendo a quantificação realizada através de cromatografia líquida de alta eficiência. A AFB1 foi detectada em 40% das rações em níveis de 1,0 a 19,5 μg.kg-1. A concentração de AFM1 em 36,7% de amostras de leite positivas variou de 0,010 a 0,645 μg.L-1. Somente uma amostra de leite estava acima do limite de tolerância adotado no Brasil (0,5 μg.L-1) para AFM1. Concluiu-se que as concentrações de aflatoxinas na ração e no leite foram relativamente baixas, embora a alta frequência das aflatoxinas nas amostras analisadas indique a necessidade de contínuo monitoramento a fim de prevenir a contaminação de ingredientes e rações destinadas ao gado leiteiro.
The occurrence of aflatoxin B1 (AFB1) in animal feed and aflatoxin M1 (AFM1) in raw milk was evaluated in dairy farms located in the Northeast region of São Paulo state, Brazil, from October 2005 to February 2006. The Aflatoxin analysis was performed using immunoaffinity clean-up with high performance liquid chromatography for quantification. AFB1 was found in 40% of the animal feeds at the levels of 1.0 to 19.5 μg.kg-1. The concentration of AFM1 in raw milk (36.7%) ranged from 0.010 to 0.645 μg.L-1. Only one single sample of raw milk presented values above the tolerance limit adopted in Brazil (0.5 μg.L-1) for AFM1. In conclusion, the concentrations of aflatoxins in the animal feed and milk samples studied were relatively low although the high frequency of mycotoxins in the both analysed samples indicates the necessity of continuous monitoring in order to prevent mycotoxin contamination of animal feed ingredients for dairy cattle.
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oliveira2010fooddeterminao Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Carlos Augusto Fernandes de Oliveira;Luciana Soares Sebastião;Helena Fagundes;Roice Eliana Rosim;Andrezza Maria Fernandes
Journal software - practice and experience
Year 2010
DOI
10.1590/S0101-20612010000500034
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000500034
Keywords
mycotoxins residues occurrencenutrition

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