A technique for simultaneous measurement of Ca2+, FRET fluorescence and force in intact mouse small arteries

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ID: 113393
2008
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Abstract
FRET (Forster resonance energy transfer)-based biosensor molecules are powerful tools to reveal specific molecular interactions in cells. Typically however, they are used in cultured cells that (inevitably) express different genes than their counterparts in intact organisms. In such cells it may be impossible to administer physiological stimuli and measure physiological outputs. Here, through the use of transgenic mice that express a FRET-based myosin light chain kinase (MLCK) biosensor molecule, we report a technique for dynamically observing activation and regulation of MLCK within the smooth muscle cells of intact, functioning small arteries, together with measurement of arterial force production and intracellular [Ca2+].
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zacharia2008thea Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors W. Gil Wier,M. A. Rizzo,Hema Raina,Joseph Zacharia;W. Gil Wier;M. A. Rizzo;Hema Raina;Joseph Zacharia;
Journal the journal of physiology
Year 2008
DOI
10.1113/jphysiol.2008.151522
URL
https://sciprofiles.com/publication/view/93d7976a9d492c1bd0a2277b882ba559
Keywords
resonance energy transfer fret transgenic mice small arteries mlck biosensor molecules smooth muscle cells

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