Detection of Nitric Oxide by Electron Paramagnetic Resonance Spectroscopy: Spin-Trapping with Iron-Dithiocarbamates.

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2016
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Abstract
Electron paramagnetic resonance (EPR) spectroscopy is the ideal methodology to identify radicals (detection and characterization of molecular structure) and to study their kinetics, in both simple and complex biological systems. The very low concentration and short life-time of NO and of many other radicals do not favor its direct detection and spin-traps are needed to produce a new and persistent radical that can be subsequently detected by EPR spectroscopy.In this chapter, we present the basic concepts of EPR spectroscopy and of some spin-trapping methodologies to study NO. The "strengths and weaknesses" of iron-dithiocarbamates utilization, the NO traps of choice for the authors, are thoroughly discussed and a detailed description of the method to quantify the NO formation by molybdoenzymes is provided.
Reference Key
maia2016detectionmethods Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Maia, Luisa B;Moura, José J G;
Journal methods in molecular biology (clifton, nj)
Year 2016
DOI
10.1007/978-1-4939-3600-7_8
URL
Keywords
nitrite aldehyde oxidoreductase electron paramagnetic resonance (epr) iron-dithiocarbamate nitric oxide radical spin-trap xanthine oxidoreductase

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