Imaging DNA single-strand breaks generated by reactive oxygen species using a liquid crystal-based sensor.

Clicks: 357
ID: 93797
2018
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Abstract
DNA single-strand breaks (SSBs) have attracted much interest since they are highly related to carcinogenesis and ageing. Herein, we report a new liquid crystal (LC)-based sensor for the detection of DNA SSBs generated by reactive oxygen species (ROS) created from the Fenton reaction. The adsorption of single-stranded DNA (ssDNA) onto the cationic surfactant-laden aqueous/LC interface interferes with the surfactant layer, inducing a planar orientation of the LCs. However, the DNA SSBs generated by the Fenton reaction-produced ROS result in a decrease of the electrostatic interactions between the ssDNA and cationic surfactant molecules, causing rearrangement of the surfactant layer and reorientation of the LCs back to a homeotropic alignment. The changes in orientation of the LCs caused by the DNA SSBs are simply converted and observed as a shift from a bright optical image of the LCs to a dark one under a polarized light microscope. With this simple LC-based approach, the DNA SSBs could be detected more effectively and rapidly without any complex instrumentation or intricate processes. Therefore, our research provides a novel strategy for the detection of DNA damage as well as better insight into the DNA-damaging process.
Reference Key
kim2018imaginganalytical Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Kim, Hyeong Jin;Jang, Chang-Hyun;
Journal analytical biochemistry
Year 2018
DOI
S0003-2697(18)30210-0
URL
Keywords
fenton reaction reactive oxygen species (ros) 4-cyano-4′-pentylbiphenyl (5cb) dna single-strand breaks (ssbs) liquid crystal (lc) orientational transition

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