Systematic analyses of factors required for adhesion of serovar Typhimurium to corn salad ().

Clicks: 217
ID: 93658
2020
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #105 of 150 articles by views in Applied and environmental microbiology

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 150 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
is a foodborne pathogen often leading to gastroenteritis and is commonly acquired by consumption of contaminated food of animal origin. However, frequency of outbreaks linked to the consumption of fresh or minimally processed food of non-animal origin is increasing. New infection routes of by vegetables, fruits, nuts and herbs have to be considered. This leads to special interest in interactions with leafy products, e.g. salads, that are mainly consumed in minimally processed form. The attachment of to salad is a crucial step in contamination, but little is known about the bacterial factors required and mechanisms of adhesion. possesses a complex set of adhesive structures whose functions are only partly understood. Potentially, may deploy multiple adhesive strategies for adhering to various salad species, and other vegetables. Here, we systematically analyzed the contribution of the complete adhesiome, of LPS, and of flagella-mediated motility of serovar Typhimurium (STM) in adhesion to (corn salad). We deployed a reductionist, synthetic approach to identify factors involved in the surface binding of STM to leaves of corn salad with particular regard to the expression of all known adhesive structures using the Tet-on system. This work reveals the contribution of Saf fimbriae, type 1 secretion system-secreted BapA, an intact LPS, and flagella-mediated motility of STM in adhesion to corn salad leaves.Transmission of gastrointestinal pathogens by contaminated fresh produce is of increasing relevance to human health. However, the mechanisms of contamination of, persistence on, and transmission by fresh produce are poorly understood. We investigated the contribution of the various adhesive structures of STM to the initial event in transmission, i.e. binding to the plant surface. A reductionist system was used that allowed the experimentally controlled surface expression of individual adhesive structures, and the analyses of the contribution to binding to leave surfaces of corn salat under laboratory conditions. The model systems allowed the determination of the relative contribution of fimbrial and non-fimbrial adhesins, the type III secretion systems, the O-antigen of lipopolysaccharide, the flagella and chemotaxis of STM to binding to corn salad leaves. Based on these data, future work could reveal the mechanism of binding, and the relevance of interaction under agricultural conditions.
Reference Key
elpers2020systematicapplied Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Elpers, Laura;Kretzschmar, Juliane;Nuccio, Sean-Paul;Bäumler, Andreas J;Hensel, Michael;
Journal Applied and environmental microbiology
Year 2020
DOI
AEM.02757-19
URL
Keywords
beef, performance metric, standards, processing, safety

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.