Cryopreservation of Pineapple Shoot Tips by the Droplet Vitrification Technique.

Clicks: 420
ID: 93456
2018
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #12 of 139 articles by views in methods in molecular biology (clifton, nj)

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 139 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Cryopreservation is a technique that allows the conservation of many species for long periods. Among the protocols used for cryopreservation, droplet vitrification has shown efficient results in preserving shoot tips of various wild and cultivated pineapple genotypes. The method consists of extraction of shoot tips from plants grown in vitro, dehydration for a period of 48 h in a preculture medium supplemented with a high concentration of sucrose, treatment in a plant vitrification solution (PVS2), and immersion in liquid nitrogen. The method described in this chapter has produced survival and regeneration indices of around 70%, depending on the genotype and physiological conditions of the initial explants. The objective of this chapter is to describe in detail a droplet vitrification protocol for shoot tips that is easy to perform for cryopreservation of pineapple germplasm.
Reference Key
souza2018cryopreservationmethods Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Souza, Fernanda Vidigal Duarte;de Souza, Everton Hilo;Kaya, Ergun;de Jesus Vieira, Lívia;da Silva, Ronilze Leite;
Journal methods in molecular biology (clifton, nj)
Year 2018
DOI
10.1007/978-1-4939-8594-4_18
URL
Keywords
conservation ananas comosus plant vitrification solution

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.