Phytochemical Profiles and In Vitro Immunomodulatory Activity of Ethanolic Extracts from L.

L., family Rubiaceae, is a widely spread species in the genus. The herb of is part of folk remedies and dietary supplements. In this study, we analyzed the chemical composition and immunomodulatory activities of herb ethanolic extracts obtained from the plant material by maceration with 20%, 60% or 96% ethanol. The contents of hydroxycinnamic acid derivatives, flavonoids and polyphenols were determined spectrophotometrically, with extractives and polysaccharides quantified gravimetrically. The qualitative composition was studied using UHPLC-DAD-MS/MS analysis; isolation not previously described in quercetin rhamnoglucoside was carried out through column chromatography, and the immunomodulatory activity of extracts was determined in the reaction of lymphocyte blast transformation. Major constitutes of extracts were iridoids, i.e., monotropein, 10-desacetylasperulosidic acid and asperulosidic acid; -hydroxybenzoic acid; hydroxycinnamic acid derivatives, i.e., 3--caffeoylquinic, 5--caffeoylquinic, 3,4--dicaffeoylquinic, 3,5--dicaffeoylquinic, 4,5-O-dicaffeoylquinic acids and caffeic acid derivatives; flavonoids, i.e., rutin, quercetin 3--rhamnoglucoside-7--glucoside, and isorhamnetin 3--glucorhamnoside. Significantly, quercetin 3--rhamnoglucoside-7--glucoside was first isolated and identified in species so far investigated. All herb ethanolic extracts stimulate the transformational activity of immunocompetent blood cells, with 96% ethanolic extract being the most active. The data obtained necessitate further research into the mechanisms of immunomodulatory activity of extracts from herb.