Simultaneous determination of cereal monosaccharides, xylo- and arabinoxylo-oligosaccharides and uronic acids using HPAEC-PAD.

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ID: 89482
2020
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Abstract
Xylo- and arabinoxylo-oligosaccharides (XOS and AXOS) are of interest for their prebiotic activity. The production of these oligomers might be accompanied with monosaccharides. The measurement of both oligosaccharides and monosaccharides usually requires two methods. The current work presents an HPAEC-PAD method based on gradient elution of aqueous solvents sodium hydroxide and sodium acetate, in contrast to conventional isocratic elution, for the simultaneous separation of 16 standards of monosaccharides, xylo-oligosaccharides, arabinoxylo-oligosaccharides and uronic acids using CarboPac PA 200 column. The presented method showed a stable baseline and high-resolution separation of the standards. The method showed acceptable accuracy and precision. Limits of Detection and Quantitation (LOD and LOQ) were estimated for all the standards. The method was applied to measure the activity of a commercial endoxylanase on wheat bran; a steady release of xylose monosaccharide was observed. Enzyme action on oligosaccharide standards showed a preference for the larger oligosaccharides.
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alyassin2020simultaneousfood Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Alyassin, M;Campbell, G M;Masey O'Neill, H;Bedford, M R;
Journal Food chemistry
Year 2020
DOI
S0308-8146(20)30068-6
URL
Keywords
wheat bran arabinoxylo-oligosaccharides (axos) hpaec-pad oligosaccharide separation xylanase activity xylo-oligosaccharides (xos)

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