Evaluation of immuno-modulating effect of recombinant heat shock protein 40 of in mice.

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ID: 87778
2019
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Abstract
The present study was aimed to evaluate the immuno-modulatory effect of -specific recombinant HSP40 (rDnaJ) when co-immunized with rOmp22 in mice. For this, of was cloned, expressed in and purified to homogeneity using Ni-NTA agarose columns. Three groups of mice ( = 6 in each group) were used in the study. The control group was immunized with rOmp22 alone, while group 1 mice were injected subcutaneously with rOmp22 along with conventional adjuvants (FCA, FIA), and group 2 mice with rOmp22 mixed with rDnaJ. IgG isotype (IgG1 and IgG2a) response to rOmp22 immunization was evaluated by enzyme-linked immunosorbent assay which was found to be directed towards the cell-mediated arm of immune system (CMI) in group 2 mice in which rOmp22 was co-immunized with rDnaJ. Expression profiling of IL-4 and IL-12 was checked in all the groups by qRT PCR. IL12 mRNA was up-regulated to a greater extent in group2 mice, suggesting that the CMI arm of immune system was stimulated. Hence, it was concluded that CMI response against rOmp22 is stimulated to a greater extent in mice when co-immunized with rDnaJ.
Reference Key
minhas2019evaluation3 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Minhas, Priyanka;Sunil Kumar, B V;Verma, Ramneek;
Journal 3 Biotech
Year 2019
DOI
10.1007/s13205-019-1905-3
URL
Keywords
heat shock proteins dnaj brucella abortus immuno-modulatory romp22

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