Bacteria isolated from abscesses of small ruminants inspected in the semiarid region of Brazil

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2016
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Abstract
Loss in the supply chain of small ruminants owing to condemnations of carcasses in the abattoirs and slaughterhouses is common in northeastern Brazil. This study aims to identify bacterial agents, including Mycobacterium spp., in the abscesses found in the postmortem analysis of the carcasses of sheep and goats bred in northeastern Brazil. Our analysis involved 679 goats and 1,838 sheep carcasses. Abscess samples were extracted and inoculated on blood agar and Lowenstein Jensen with pyruvate or glycerol for bacterial isolation. We then performed polymerase chain reaction of the hps 65 gene; samples positive for Mycobacterium spp. were subjected to DNA sequencing. Relative frequencies of abscesses in goats and sheep were 5.44 and 3.26%, respectively. Microbiological analysis revealed 87.7% bacterial growth in the inoculated samples. Among these, Corynebacterium pseudotuberculosis represented 67.7% of the isolates. We observed 1.9% mycobacteria growth in the abscess samples inoculated on Lowenstein-Jensen medium. PCR of DNA extracted from abscesses samples showed amplification of 0.9% of samples. After sequencing, Mycobacterium spp. isolate was identified as M. novocastrense. C. pseudotuberculosis was the main agent responsible for the formation of abscesses in the examined animals, and we did not identify any species of the M. tuberculosis complex in the examined small ruminants.
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Authors Silva, Wellington Erasmo Lima e;Gouveia, Gisele Veneroni;Sá, Maria da Conceição Aquino de;Gouveia, João José de Simoni;Peixoto, Rodolfo de Moraes;Riet-Correa, Franklin;Veschi, Josir Laine Aparecida;Costa, Mateus Matiuzzi da;
Journal semina: ciências agrárias
Year 2016
DOI
DOI not found
URL
http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/21508
Keywords
Information technology Agriculture (General) environmental effects of industries and plants renewable energy sources environmental sciences nutrition. foods and food supply plant culture political science political science (general) agricultural industries Agriculture (General) sheep isolation pcr goat inspection

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