Development of a lateral flow aptamer assay strip for facile identification of theranostic exosomes isolated from human lung carcinoma cells.

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ID: 85837
2020
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Abstract
Exosomes are Extracellular Vesicles (EV) that own unique structural features and functions and have gradually become the hot research spot in recent years. The tumor-derived exosomes contain various types of useful biological information, and medical identification of exosomes relied on the specific characterization of membrane surface proteins. In this study, in order to rapidly identify non-small cell lung cancer (NSCLC)-derived exosomes, based on an aptamer against CD63 protein on exosome membrane, a low cost lateral flow aptamer assay (LFAA) test strip using nanogold particles as visualization probes was successfully developed for facile identification of A549 exosomes isolated from human lung carcinoma cells diluted from 6.4 × 10 particles/mL herein.
Reference Key
yu2020developmentanalytical Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Yu, Qiang;Zhao, Qu;Wang, Sai;Zhao, Shuai;Zhang, Shan;Yin, Yingai;Dong, Yiyang;
Journal analytical biochemistry
Year 2020
DOI
S0003-2697(19)31083-8
URL
Keywords
non-small cell lung cancer (nsclc) aptamer exosomes lateral flow aptamer assay (lfaa)

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