Evaluation of resazurin microtiter plate assay and HPLC- photodiode array analysis of the roots of Asparagus adscendens.

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ID: 83607
2018
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Abstract
Asparagus adscendens Roxb. (Asparagaceae), is native to the Himalayas. The present study, for the first time, was undertaken to explore the antimicrobial potential, to determine the minimum inhibitory concentration (MIC) values of the methanol extract of the roots of A. adscendens and its solid-phase extraction (SPE) fractions using resazurin microtitre assay against Gram-positive and negative bacterial-registered strains and to carry out HPLC-photodiode array analysis of the SPE fractions. The methanol extract and all SPE exhibited considerable level of antibacterial potential against Gram-positive bacteria (MIC: 2.5-0.009 mg/mL) than against Gram-negative bacteria (MIC: 1.25-2.5 mg/mL). The use of microtitre plates has the advantage of lower cost, fast and quantitative results. Like other Asparagus species, the presence of phenolic compounds in all SPE fractions was evident in the HPLC-PDA data.
Reference Key
khan2018evaluationnatural Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Khan, Kashif Maqbool;Nahar, Lutfun;Mannan, Abdul;Arfan, Muhammad;Khan, Ghazanfar Ali;Hobbs, Glyn;Sarker, Satyajit D;
Journal Natural product research
Year 2018
DOI
10.1080/14786419.2017.1353509
URL
Keywords
spe asparagaceae mic rema asparagus adscendens hplc-pda

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