Cytokine Regulation from Human Peripheral Blood Leukocytes Cultured with Silver Doped Bioactive Glasses Microparticles.

Clicks: 393
ID: 76880
2019
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Abstract
Bioactive glasses (BG) applications include tissue engineering for bone regeneration, coating for implants, and scaffolds for wound healing. BG can be conjugated to ions like silver, which might add some antimicrobial properties to this biomaterial. The immunomodulatory activity of ion-doped bioactive glasses particles was not investigated before. The aim of this work was to evaluate the cytotoxic and immunomodulatory effect of BG and silver-doped bioactive glass (BGAg) in human peripheral blood cells. BG and BGAg samples belonging to the system 58SiO(36-x)CaO6POxAgO, where x = 0 and 1 mol%, respectively, were synthesized via sol-gel method and characterized. Cytotoxicity, modulation of cytokine production (TNF-, IL-1, IL-6, IL-4, and IL-10), and oxidative stress response were investigated in human polymorphonuclear cells (PMNs) and peripheral blood mononuclear cells (PBMCs) cultures. Cell viability in the presence of BG or BGAg was concentration-dependent. In addition, BGAg presented higher PBMCs toxicity (LC50 = 0.005%) when compared to BG (LC50 = 0.106%). Interestingly, interleukin4 was produced by PBMCs in response to BG and BGAg in absence of phytohemagglutinin (PHA) and did not modulate PHA-induced cytokine levels. Subtoxic concentrations (0.031% for BG and 0.0008% for BGAg) did not change other cytokines in PBMCs nor reactive oxygen species (ROS) production by PMN. However, BG and BGAg particles decreased zymosan-induced ROS levels in PMN. Although ion incorporation increased BG cytotoxicity, the bioactive glass particles demonstrated a anti-inflammatory potencial. Future studies are needed to clarify the scavenger potential of the BG/BGAg particles/scaffolds as well as elucidate the effect of the anti-inflammatory potential in modulating tissue growth .
Reference Key
de-lima2019cytokinebiomed Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors de Lima, Jefferson Muniz;Pinheiro Ferreira, Edlainne;Bonan, Roberta Ferreti;Silva-Teixeira, David Nascimento;Goulart, Luiz Ricardo;de Souza, Joelma Rodrigues;de Medeiros, Eliton Souto;Bonan, Paulo Rogério Ferreti;Castellano, Lúcio Roberto Cançado;
Journal BioMed research international
Year 2019
DOI
10.1155/2019/3210530
URL
Keywords
therapeutics intensive care chikungunya fever flebogamma dif intravenous antibodies

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