An ultrasensitive electrochemical sensor for 17β-estradiol using split aptamers.

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ID: 76636
2019
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Abstract
Herein, a simple electrochemical sensor is proposed for 17β-estradiol (E2) determination. In this sensing platform, split DNA aptamers for E2 were applied as recognizing agents. In the presence of E2, split aptamers are bound to E2 and establish split1-E2-split2 complex as a bridge on the surface of electrode. This physical bar leads to ultrasensitive detection of E2. This aptasensor is capable of recognizing E2 within 30 min without complicated procedures and expensive equipment. This sensing approach indicated a wide linear range with detection limits of 0.5 pM and 0.7 pM for E2 in tap water and milk samples, respectively. Simplicity and high specificity of the proposed electrochemical aptasensor make it an ideal sensing platform for ultra-low detection of other targets in complicated samples by replacement of split DNA aptamers.
Reference Key
nameghi2019ananalytica Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Nameghi, Morteza Alinezhad;Danesh, Noor Mohammad;Ramezani, Mohammad;Alibolandi, Mona;Abnous, Khalil;Taghdisi, Seyed Mohammad;
Journal analytica chimica acta
Year 2019
DOI
S0003-2670(19)30310-1
URL
Keywords
17β-estradiol electrochemical sensing platform milk sample split dna aptamers

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