Setup of Quantitative PCR for Oral spp. Evaluation in Celiac Disease Diagnosis.

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ID: 74534
2019
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Abstract
Coeliac disease (CD) is a multifactorial autoimmune disorder and gut dysbiosis contributes to its pathogenesis. We previously profiled by 16S rRNA sequencing duodenal and oropharyngeal microbiomes in active CD (a-CD), gluten-free diet (GFD) patients, and controls (CO) and found significantly higher levels of spp., with pro-inflammatory activities, in a-CD patients than in the other two groups. In this study, we developed a fast and simple qPCR-based method to evaluate the abundance of the oral spp. and the diagnostic performances of the test in CD diagnosis. The spp. abundances detected by quantitative PCR (qPCR) were: CO = 0.14, GFD = 0.15, a-CD = 2.08, showing a similar trend to those previously measured by next generation sequencing (NGS). In particular, spp. values obtained by both methods were significantly higher ( < 0.001) in a-CD than in the other two groups GFD and CO-the latter almost overlapping. We calculated by ROC curve analysis the threshold of 1.12 ng/μL of spp. to discriminate between CO+GFD and a-CD patients with 100% and 96.7% of diagnostic sensitivity and specificity, respectively. In conclusion, our data, if confirmed in other cohorts, suggest the q-PCR evaluation of oral spp. could be a fast and simple method to assess CD-associated dysbiosis for diagnostic purposes.
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Authors Maria Valeria Esposito, Carmela Nardelli, Ilaria Granata, Chiara Pagliuca, Valeria D'Argenio, Ilaria Russo, Mario Rosario Guarracino, Paola Salvatore, Giovanna Del Vecchio Blanco, Carolina Ciacci, Lucia Sacchetti
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Journal Diagnostics (Basel, Switzerland)
Year 2019
DOI
E12
URL
Keywords
celiac disease qpcr diagnostic marker oral microbiome neisseria flavescens azole resistance diagnostics aspergillus fumigatus pyrosequencing antifungal drug resistance cyp51a depression schizophrenia polyunsaturated fatty acids dietary fatty acids microbiota-brain communication muscle aerobic exercise creatine concurrent exercise strength loss thermodynamics astrobiology prebiotic chemistry flory-schulz distribution rna world chemical kinetics linear step-growth polymerization nonenzymatic polymerization origins of life locomotion topology walking insect arthropoda friction leg biotribology contact mechanics joints

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