Bisphenol A impairs follicle growth, inhibits steroidogenesis, and downregulates rate-limiting enzymes in the estradiol biosynthesis pathway.

Clicks: 715
ID: 74044
2011
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #1 of 128 articles by views in toxicological sciences : an official journal of the society of toxicology

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 128 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Bisphenol A (BPA) is used as the backbone for plastics and epoxy resins, including various food and beverage containers. BPA has also been detected in 95% of random urine samples and ovarian follicular fluid of adult women. Few studies have investigated the effects of BPA on antral follicles, the main producers of sex steroid hormones and the only follicles capable of ovulation. Thus, this study tested the hypothesis that postnatal BPA exposure inhibits antral follicle growth and steroidogenesis. To test this hypothesis, antral follicles isolated from 32-day-old FVB mice were cultured with vehicle control (dimethyl sulfoxide [DMSO]), BPA (4.4-440 μM), pregnenolone (10 μg/ml), pregnenolone + BPA 44 μM, and pregnenolone + BPA 440 μM. During the culture, follicles were measured for growth daily. After the culture, media was subjected to ELISA for hormones in the estradiol biosynthesis pathway, and follicles were processed for quantitative real-time PCR of steroidogenic enzymes. The results indicate that BPA (440 μM) inhibits follicle growth and that pregnenolone cotreatment was unable to restore/maintain growth. Furthermore, BPA 44 and 440 μM inhibit progesterone, dehydroepiandrosterone, androstenedione, estrone, testosterone, and estradiol production. Pregnenolone cotreatment was able to increase production of pregnenolone, progesterone, and dehydroepiandrosterone and maintain androstenedione and estrone levels in BPA-treated follicles compared with DMSO controls but was unable to protect testosterone or estradiol levels. Furthermore, pregnenolone was unable to protect follicles from BPA-(44-440 μM) induced inhibition of steroidogenic enzymes compared with the DMSO control. Collectively, these data show that BPA targets the estradiol biosynthesis pathway in the ovary.
Reference Key
peretz2011bisphenoltoxicological Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Peretz, Jackye;Gupta, Rupesh K;Singh, Jeffrey;Hernández-Ochoa, Isabel;Flaws, Jodi A;
Journal toxicological sciences : an official journal of the society of toxicology
Year 2011
DOI
10.1093/toxsci/kfq319
URL
Keywords
NMR HPLC sodium carboxymethyl cellulose SEM metabolic disease high-performance liquid chromatography gst ampk nox mtor traditional chinese medicine thin layer chromatography glutathione-s-transferase nf-κb mammalian target of rapamycin nuclear magnetic resonance dimethyl sulfoxide fcs pbs fetal calf serum peroxisome proliferator-activated receptor gamma tlc bovine serum albumin dmso bmp natural product bsa anova srebp pparγ dmem phosphate buffered saline lbd 3-isobutyl-1-methylxanthine amp-activated kinase atcc american type culture collection badge cmcna dulbecco's modified eagle's medium ec(50) egfp ibmx mef nadph-dependent oxidase nbs peroxisome proliferator-activated receptor rxr spf tcm tr-fret analysis of variance bisphenol a diglycidyl ether bone morphogenic protein 4 effective concentration 50% enhanced green fluorescent protein ligand-binding domain mouse embryonic fibroblasts newborn bovine serum nuclear factor κb retinoic x receptor specific pathogen free standard error of the mean sterol regulatory element binding protein time-resolved fluorescence resonance energy transfer

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.