Improved methods for the differentiation of hypothalamic vasopressin neurons using mouse induced pluripotent stem cells.

Clicks: 362
ID: 69643
2019
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #10 of 38 articles by views in Stem cell research

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
High differentiation efficiency is one of the most important factors in developing an in vitro model from pluripotent stem cells. In this report, we improved the handling technique applied to mouse-induced pluripotent stem (iPS) cells, resulting in better differentiation into hypothalamic vasopressin (AVP) neurons. We modified the culture procedure to make the maintenance of iPS cells in an undifferentiated state much easier. Three-dimensional floating culture was demonstrated to be effective for mouse iPS cells. We also improved the differentiation method with regards to embryology, resulting in a greater number of bigger colonies of AVP neurons differentiating from mouse iPS cells. Fgf8, which was not used in the original differentiation method, increased iPS differentiation into AVP neurons. These refinements will be useful as a valuable tool for the modeling of degenerative disease in AVP neurons in vitro using disease-specific iPS cells in future studies.
Reference Key
mitsumoto2019improvedstem Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Mitsumoto, Kazuki;Suga, Hidetaka;Sakakibara, Mayu;Soen, Mika;Yamada, Tomiko;Ozaki, Hajime;Nagai, Takashi;Kano, Mayuko;Kasai, Takatoshi;Ozone, Chikafumi;Ogawa, Koichiro;Sugiyama, Mariko;Onoue, Takeshi;Tsunekawa, Taku;Takagi, Hiroshi;Hagiwara, Daisuke;Ito, Yoshihiro;Iwama, Shintaro;Goto, Motomitsu;Banno, Ryoichi;Arima, Hiroshi;
Journal Stem cell research
Year 2019
DOI
S1873-5061(19)30202-8
URL
Keywords
differentiation maintenance hypothalamus avp neuron mouse ips cells

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.