High-performance liquid chromatography and gas chromatography to set the analysis method of stearoyl lactylate, a food emulsifier.
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2019
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Abstract
Stearoyl lactylates (SLs, E481/E482) and their sodium and calcium salts are emulsifiers commonly used as food additives. This study was performed to set the analytical method of SLs in Korea using high-performance liquid chromatography (HPLC) and gas chromatography (GC). A new HPLC method using C18 column with water and methanol as the eluents, and a new GC method using DB-1 column with He as the carrier gas, were developed and were compared with previously reported analytical methods. The new HPLC and GC methods indicated a coefficient of determination (r) of 0.999 in the calibration curves. The detection and quantification limits are 0.26 and 0.78 μg/kg for the HPLC method, respectively, and 16.54 and 50.12 μg/kg for the GC method, respectively. The new HPLC and GC methods indicated precision of 0-2.0%, and accuracy of 92.7-108.5%. Therefore, they can be applied in numerous food samples to determine the level of SLs.
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park2019highperformancefood
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| Authors | Park, Juhee;Kim, Hyondeog;Hong, Seungran;Suh, Hee-Jae;Lee, Chan; |
| Journal | Food science and biotechnology |
| Year | 2019 |
| DOI |
10.1007/s10068-019-00629-1
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