Intracellular K and water content in human blood lymphocytes during transition from quiescence to proliferation.

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ID: 67811
2019
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Abstract
Many evidence shows that K ions are required for cell proliferation, however, changes in intracellular K concentration during transition of cells from quiescence to cycling are insufficiently studied. Here, we show using flame emission assay that a long-term increase in cell K content per g cell protein is a mandatory factor for transition of quiescent human peripheral blood lymphocytes (PBL) to proliferation induced by phytohemagglutinin, phorbol ester with ionomycin, and anti-CD3 antibodies with interleukin-2 (IL-2). The long-term increase in K content is associated with IL-2-dependent stage of PBL activation and accompanies the growth of small lymphocytes and their transformation into blasts. Inhibition of PBL proliferation with drugs specific for different steps of G0/G1/S transit prevented both blast-transformation and an increase in K content per cell protein. Determination of the water content in cells by measuring the density of cells in the Percoll gradient showed that, unlike the K content, the concentration of K in cell water remains unchanged, since water and K change in parallel. Correlation of proliferation with high cell K and water content has been confirmed by the data obtained in comparative study of PBL and permanently cycling Jurkat cells. Our data suggest that K is important for successful proliferation as the main intracellular ion that participates in regulation of cell water content during cell transition from quiescence to proliferation. We concluded that high K content in cells and the associated high water content is a characteristic feature of proliferating cells.
Reference Key
marakhova2019intracellularscientific Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Irina Marakhova, Valentina Yurinskaya, Nikolay Aksenov, Valeriy Zenin, Alla Shatrova, Alexey Vereninov
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Journal Scientific reports
Year 2019
DOI
10.1038/s41598-019-52571-1
URL
Keywords
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