Enhanced scale and scope of genome engineering and regulation using CRISPR/Cas in Saccharomyces cerevisiae.

Clicks: 380
ID: 67586
2019
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Abstract
Although only 6 years old, the CRISPR system has blossomed into a tool for rapid, on-demand genome engineering and gene regulation in Saccharomyces cerevisiae. In this minireview, we discuss fundamental CRISPR technologies, tools to improve the efficiency and capabilities of gene targeting, and cutting-edge techniques to explore gene editing and transcriptional regulation at genome scale using pooled approaches. The focus is on applications to metabolic engineering with topics including development of techniques to edit the genome in multiplex, tools to enable large numbers of genetic modifications using pooled single-guide RNA libraries and efforts to enable programmable transcriptional regulation using endonuclease-null Cas enzymes.
Reference Key
deaner2019enhancedfems Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Deaner, Matthew;Alper, Hal S;
Journal fems yeast research
Year 2019
DOI
foz076
URL
Keywords
metabolic engineering synthetic biology crispr saccharomyces cerevisiae cas9 cpf1 dcas9

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