Extra-cellular isoamylase production by Rhizopus oryzae in solid-state fermentation of agro wastes

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ID: 66791
2011
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Ranked #7 of 64 articles by views in brazilian archives of biology and technology

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Abstract
Extra-cellular isoamylase was produced by Rhizopus oryzae PR7 in solid-state fermentations of various agro wastes, among which millet, oat, tapioca, and arum (Colocasia esculenta) showed promising results. The highest amount of enzyme production was obtained after 72 h of growth at 28°C. The optimum pH for enzyme production was - 8.0. Among the various additives tested, enzyme production increased with ions such as Ca2+, Mg2+ and also with cysteine, GSH, and DTT. The enzyme synthesis was reduced in the presence of thiol inhibitors like Cu2+ and pCMB. The surfactants like Tween-40, Tween-80 and Triton X-100 helped in enhancing the enzyme activity. The production could be further increased by using the combinations of substrates. The ability to produce high amount of isoamylase within a relatively very short period and the capability of degrading wastes could make the strain suitable for commercial production of the enzyme.
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ghosh2011extracellularbrazilian Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Ghosh, Barnita;Ray, Rina Rani;
Journal brazilian archives of biology and technology
Year 2011
DOI
DOI not found
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500003
Keywords
Microbiology Medicine (General) Medicine Biotechnology Science

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