Ether Hydrolysis, Ether Thiolysis and the Catalytic Power of Etherases in the Disassembly of Lignin.

Clicks: 263
ID: 64184
2019
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #17 of 73 articles by views in Biochemistry

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
The recycling of much of the carbon in Nature depends on the breakdown of polymers in woody matter, notably cellulose (a polyacetal) and lignin (a polyether). Here, we show that equilibrium favors ether hydrolysis in water, but that the rates of hydrolysis of ethers are too slow to measure in neutral solution except at temperatures approaching the critical point of water. Circumventing that obstacle, glutathione-dependent etherases from white-rot fungi are known to employ the thiolate group of glutathione to attack guaiacyl ethers. The present experiments at elevated temperatures indicate that thioglycolate attacks diethyl ether in water-in the absence of enzymes-with a rate constant of 6 x 10^-11 M^-1 s^-1 at 25 °, and that thiolysis is strongly favored thermodynamically, with a K(v)eq value of 2.5 x 10^6 (ΔG ~ -8.7 kcal/mol). Compared with the rate of nonenzymatic thiolysis, the lignin-degrading etherases LigE and LigF produce 10^15-fold rate enhancements, among the largest that have been observed for an enzyme acting on two substrates. As in other enzymatic displacement reactions, one function of these enzymes' active sites is presumably to remove the substrates from constraints on their reactivity imposed by solvent water.
Reference Key
lewis2019etherbiochemistry Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Lewis, Charles A;Wolfenden, Richard;
Journal Biochemistry
Year 2019
DOI
10.1021/acs.biochem.9b00698
URL
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.