Novel protocol optimized for microalgae lutein used as food additives.

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ID: 63638
2019
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Abstract
Lutein available in the current market is derived from marigold petals. However, extensive studies showed that microalgae are rich in lutein content and potentially exploitable for its dietary and other industrial applications. In this study, microwave assisted binary phase solvent extraction method (MABS) was the novel protocol being developed and optimized to achieve maximum lutein recovery from microalgae Scenedesmus sp. biomass. Results showed that 60% potassium hydroxide solution with acetone in the ratio of 0.1 (ml/ml) was the ideal binary phase solvent composition. Empirical model developed using response surface methodology revealed highest lutein content can be recovered through MABS extraction method at 55 °C treatment temperature, 36 min in extraction time, 0.7 (mg/ml) for biomass to solvent ratio, 250 Watt microwave power and 250 rpm stirring speed. This optimized novel protocol had increased the amount of lutein recovered by 130% and shorten the overall extraction time by 3-folds.
Reference Key
low2019novelfood Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Low, K L;Idris, A;Mohd Yusof, N;
Journal Food chemistry
Year 2019
DOI
S0308-8146(19)31756-X
URL
Keywords
Microwave extraction microalgae lutein binary phase solvent respond surface methodology

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