Oligochitosan modified albumin as plasmid DNA delivery vector: endocytic trafficking, polyplex fate, in vivo compatibility.

Clicks: 326
ID: 60032
2019
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Ranked #73 of 200 articles by views in International journal of biological macromolecules

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Abstract
Cationic macromolecules condense DNA into small nanoparticles and form polyplex. The composition of the polyplex determines the endocytic process, the intracellular routing and the fate of the polyplex. Previously, oligochitosan-modified vectors with different protein moieties are used as gene delivery vector and the types of protein moiety can influence the endosome escape ability and transfection efficiency. Among the modified vectors, oligochitosan-modified bovine serum albumin (BSA) showed 90% transfection efficeincy compared to the modified zein and ovalbumin. These data encouraged us to investigate the mechanism of internalization involved in the superior transfection efficiency of modified BSA/ plasmid polyplex. The effect of specific endocytic inhibitors was studied in two adherent cell lines. The caveolae-mediated and lipid-mediated pathways play a significant role in the polyplex internalization. Next, a colocation of polyplex with lysosome was investigated in the presence of LysoTracker using confocal microscopy. Up to 70% of polyplex successfully escaped the lysosome without degradation. Four non-adherent cell lines showed above than 60% transfection efficiency at an optimized vector/plasmid ratio. Moreover, no significant hemolytic effect was observed up to 500 μg/mL of cationic BSA, indicating no detectable cell membrane disruption. Overall, the hybrid biomacromolecule showed good intracellular delivery and safety in a mice model.
Reference Key
kumari2019oligochitosaninternational Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Kumari, Monika;Liu, Chi-Hsien;Wu, Wei-Chi;
Journal International journal of biological macromolecules
Year 2019
DOI
S0141-8130(19)36095-7
URL
Keywords
albumin gene delivery trafficking oligochitosan albumin gene delivery trafficking oligochitosan

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