Enzyme-free amplified and ultrafast detection of aflatoxin B using dual-terminal proximity aptamer probes.

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ID: 59968
2019
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Abstract
Aptamer probes provide an opportunity for achieving rapid and on-site detection of food contaminants. Herein, we proposed a general design strategy for aptamer probes enabling enzyme-free amplified, ultrafast and one-test tube homogeneous detection of aflatoxin B (AFB). The key feature of the aptamer probe is designed with dual-terminal proximity structures, allowing the binding of one molecule to light up two fluorophores, leading to enzyme-free amplification and a remarkable improvement of signal to background ratio and sensitivity for AFB detection. This aptamer probe could accommodate quick response to AFB, and the detection process could be finished within 1 min, ranking one of the quickest assays for AFB. AFB detection of broad bean paste and peanut oil conferred satisfactory recoveries ranging from 90.3% to 114.8%. Contributed to the generality and simplicity of the design strategy, this structure-switching probe could potentially act as a general platform of on-site detection for food safety.
Reference Key
xia2019enzymefreefood Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Xia, Xuhan;Wang, Yuxi;Yang, Hao;Dong, Yi;Zhang, Kaixiang;Lu, Yunhao;Deng, Ruijie;He, Qiang;
Journal Food chemistry
Year 2019
DOI
S0308-8146(19)30065-2
URL
Keywords
aptamer rapid detection aflatoxin b(1) fluorescence resonance energy transfer homogeneous analysis nucleic acids probe

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