Multicolored Prestained Standard Protein Marker Generation Using a Variety of Remazol Dyes for Easy Visualization of Protein Bands During SDS-PAGE.

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ID: 599
2018
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Ranked #7 of 139 articles by views in methods in molecular biology (clifton, nj)

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Abstract
Isolation and detection of a specific protein from a complex mixture of proteins using molecular sieves provided by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used technique in molecular biology and biochemistry. The detection is achieved usually by running prestained standard markers along with the sample protein mixture. Multicolored standard markers are preferred compared to fluorescent labeled or single colored markers as it allows easy visualization of separation of a specific protein band during SDS-PAGE. In addition, these colored markers show evidence for the transfer of protein bands on the membrane in western blotting technique. This review describes a very simple, inexpensive and easy method of prestaining specific proteins with different colors using a variety of Remazol dyes.
Reference Key
kumar2018multicolored Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Kumar, Gaurav;
Journal methods in molecular biology (clifton, nj)
Year 2018
DOI
10.1007/978-1-4939-8745-0_3
URL
Keywords Keywords not found

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