Preparation of gold-labeled antibody probe and its use in immunochromatography assay for detection of aflatoxin B1.

Clicks: 319
ID: 59374
2005
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #25 of 417 articles by views in International journal of food microbiology

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 417 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Preparation of an antibody-colloidal gold probe (conjugate) specific to aflatoxin B1 (AFB1) and its use in developing a rapid AFB1 diagnostic method was presented in this paper. Monodispersional nanogold colloid was synthesized and preparation of nanogold-labeled polyclonal antibody probe to aflatoxin B1 under friendly and optimal condition. Combination of antibody with nanogold particles was also characterized by UV-visible (UV-vis) light absorption spectra, transmission electron microscopy (TEM), fluorescence spectroscopy, titers, cross reactivity and stability measurements. Furthermore, nanogold-labeled probe was used to develop an immunochromatographic (IC) method for aflatoxin B1 analysis. With this method, analysis could be completed in less than 10 min. Detection time was reduced 6-10 times comparative with ELISA. With visual observation, lower test limit was found to be around 2.5 ng/ml aflatoxin B1 standard solution, which was increased to two times of ELISA.
Reference Key
xiulan2005preparationinternational Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Xiulan, Sun;Xiaolian, Zhao;Jian, Tang;Zhou, Jun;Chu, F S;
Journal International journal of food microbiology
Year 2005
DOI
DOI not found
URL URL not found
Keywords
feed eu-nano-trfia europium label nanospheres total aflatoxin

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.