Colour-encoded lateral flow immunoassay for the simultaneous detection of aflatoxin B1 and type-B fumonisins in a single Test line.

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ID: 59359
2019
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Abstract
A multiplex Lateral Flow Immunoassay was developed based on the use of a single Test line and multicolour gold nanoparticles (GNPs) as signal reporters. Red and blue GNPs were linked to antibodies directed towards two different analytes and included in a typical lateral flow immunoassay configuration, in which the Test line was formed by the mixture of two antigens. As a result of the immunoreactions occurring at the Test zone, diverse combinations of red and blue GNPs labels were captured. Therefore, the Test line assumed different colours depending on which - and how much - analyte is present in the sample. The multiplexing capability of the 'colour-encoded assay' is illustrated by the simultaneous detection of aflatoxin B1 (AFB1) and type-B fumonisins (FMs) in wheat and food products that made with wheat. Reproducible detection of AFB1 and FMs contamination in raw and processed food was achieved with visual cut-off levels at 1 ng mL and 50 ng mL, respectively. The contaminant was identified based on the colour of the label according with a specific colour code. Furthermore, strips images were acquired by means of a common smartphone and analysed through RGB data analysis providing semi-quantitative detection of the two mycotoxins.
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di-nardo2019colourencodedtalanta Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Di Nardo, Fabio;Alladio, Eugenio;Baggiani, Claudio;Cavalera, Simone;Giovannoli, Cristina;Spano, Giulia;Anfossi, Laura;
Journal Talanta
Year 2019
DOI
S0039-9140(18)30951-2
URL
Keywords
smartphone mycotoxins blue gold nanoparticles immunochromatographic strip test multiplex detection rgb data evaluation

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