The horseradish smile: Demonstrating characteristic reactions of peroxidase in a visually appealing way.

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ID: 59230
2019
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Ranked #4 of 12 articles by views in biochemistry and molecular biology education : a bimonthly publication of the international union of biochemistry and molecular biology

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Abstract
Easy and visually appealing demonstrations are precious tools for introducing students to the study of enzymes. However, they most often involve purified enzymes and dedicated techniques. Here, we propose a set of demonstrations, which require only fresh horseradish root and consumer chemicals, to help support biochemistry and enzymology courses from high school to the undergraduate level. Horseradish root is a naturally rich source of horseradish peroxidase, an enzyme with many relevant practical applications. Slices of horseradish root are used to demonstrate the characteristic reaction of horseradish peroxidase with hydrogen peroxide, the selective inhibition of this enzyme by dilute hydrochloric acid, its chromogenic reaction with N,N'-diethyl-p-phenylenediamine, and its ability to trigger the chemiluminescent reaction of luminol. © 2019 International Union of Biochemistry and Molecular Biology, 2019.
Reference Key
panzarasa2019thebiochemistry Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Panzarasa, Guido;
Journal biochemistry and molecular biology education : a bimonthly publication of the international union of biochemistry and molecular biology
Year 2019
DOI
10.1002/bmb.21310
URL
Keywords
hydrogen peroxide enzymes chemiluminescence horseradish peroxidase demonstrations luminol

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