Characterization of Islet Leukocyte Populations in Human and Murine Islets by Flow Cytometry.

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ID: 57728
2020
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Ranked #25 of 139 articles by views in methods in molecular biology (clifton, nj)

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Abstract
An increasing body of evidence indicates that a local islet immune response is not only limited to type 1 diabetes, but also is associated with islet dysfunction in type 2 diabetes. Recently, the presence of pancreatic CD68 macrophages within islet tissues was demonstrated by RT-PCR and immunohistochemical methods. However, the precise profile and activation status of intraislet leukocytes, which are present in both murine and human islets, are poorly defined. Here, we describe a detailed flow cytometry protocol designed to analyze both human and murine islets for intraislet leukocytes and leukocyte subsets. This approach permits the simultaneous identification of multiple intraislet leukocyte subsets, as well as their activation statuses. The use of flow cytometry-based approaches will advance the field of islet biology and help to identify unique changes in the immune cell composition that accompanies pathological islet inflammation and dysfunction in type 2 diabetes.
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butcher2020characterizationmethods Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Butcher, Matthew J;Trevino, Michelle B;Imai, Yumi;Galkina, Elena V;
Journal methods in molecular biology (clifton, nj)
Year 2020
DOI
10.1007/978-1-4939-9882-1_10
URL
Keywords
type 2 diabetes flow cytometry human islets leukocytes murine islets

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