Xylose utilization stimulates mitochondrial production of isobutanol and 2-methyl-1-butanol in .

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ID: 54322
2019
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Abstract
Branched-chain higher alcohols (BCHAs), including isobutanol and 2-methyl-1-butanol, are promising advanced biofuels, superior to ethanol due to their higher energy density and better compatibility with existing gasoline infrastructure. Compartmentalizing the isobutanol biosynthetic pathway in yeast mitochondria is an effective way to produce BCHAs from glucose. However, to improve the sustainability of biofuel production, there is great interest in developing strains and processes to utilize lignocellulosic biomass, including its hemicellulose component, which is mostly composed of the pentose xylose.In this work, we rewired the xylose isomerase assimilation and mitochondrial isobutanol production pathways in the budding yeast . We then increased the flux through these pathways by making gene deletions of , , and , to develop a strain (YZy197) that produces as much as 4 g/L of BCHAs (3.10 ± 0.18 g isobutanol/L and 0.91 ± 0.02 g 2-methyl-1-butanol/L) from xylose. This represents approximately a 28-fold improvement on the highest isobutanol titers obtained from xylose previously reported in yeast and the first report of 2-methyl-1-butanol produced from xylose. The yield of total BCHAs is 57.2 ± 5.2 mg/g xylose, corresponding to ~ 14% of the maximum theoretical yield. Respirometry experiments show that xylose increases mitochondrial activity by as much as 7.3-fold compared to glucose.The enhanced levels of mitochondrial BCHA production achieved, even without disrupting ethanol byproduct formation, arise mostly from xylose activation of mitochondrial activity and are correlated with slow rates of sugar consumption.
Reference Key
zhang2019xylosebiotechnology Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Zhang, Yanfei;Lane, Stephan;Chen, Jhong-Min;Hammer, Sarah K;Luttinger, Jake;Yang, Lifeng;Jin, Yong-Su;Avalos, José L;
Journal biotechnology for biofuels
Year 2019
DOI
10.1186/s13068-019-1560-2
URL
Keywords
saccharomyces cerevisiae 2-methyl-1-butanol branched-chain higher alcohols isobutanol mitochondrial engineering xylose

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