Label-free lateral flow assay for Listeria monocytogenes by aptamer-gated release of signal molecules.

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ID: 52813
2019
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Abstract
Lateral flow assay (LFA) type of biosensors have been popular due to cost-effectiveness and easy-interpretation for instant results, most common examples of applications being pregnancy tests, food safety or medical diagnostics. There are several examples of reports with high sensitivity, including pre-concentration of the sample by magnetic pull-down. However, sensitivity and direct detection designs with aptamers has been a limiting factor for developing aptamers-based LFA assays. In this study, we report a lateral flow design based on aptamer-gated silica nanoparticles to develop high sensitivity and direct bacterial assay by shifting aptamers-target interaction to conjugation pad. Aptamer-gated silica nanoparticles-based biosensors were reported for their high sensitivity, specificity and label-free detection for small molecules and whole cells. This label-free strategy for LFA can determine L. monocytogenes in minced chicken matrix at less than 5 min with a limit of detection (LOD) of 53 cells in one mL samples.
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tasbasi2019labelfreeanalytical Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Tasbasi, B Busra;Guner, Buket C;Sudagidan, Mert;Ucak, Samet;Kavruk, Murat;Ozalp, Veli C;
Journal analytical biochemistry
Year 2019
DOI
S0003-2697(19)30763-8
URL
Keywords
nanoparticles peroxidase silica aptamers listeria

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