MicroRNA-152 Promotes Slow-Twitch Myofiber Formation via Targeting Uncoupling Protein-3 Gene.

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2019
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Abstract
The differences of pork quality characteristics among different pig breeds mainly came from the differences in myofiber type compositions. Growing evidence indicated the key role of miRNAs in myofiber specification. In the present study, we found that miR-152 is more abundant in the slow-twitch myofiber-enriched muscles. However, its role in myofiber type transformation and myogenesis is largely unknown. Overexpression of miR-152 in porcine myotubes promoted the formation of slow-twitch myofibers and myogenesis. While, inhibition of miR-152 expression showed the opposite effect to miR-152 mimics transfection. The luciferase reporter analysis confirmed that miR-152 straightly targets the 3'-untranslated region (3'-UTR) of uncoupling protein 3 () to cause its post-transcriptional inhibition in the protein level. The knockdown of by siRNA showed the similar effect of miR-152 on myofiber type transition. Furthermore, the rescue experiment in the porcine myotube transfected with miR-152 mimics or/and overexpression plasmid with or without the 3'UTR revealed that mediates the action of miR-152 in slow-twitch myofiber formation. Taken together, our findings proposed a novel molecular mechanism through which miR-152 epigenetically regulates meat quality via promoting slow-twitch myofiber formation and skeletal myogenesis.
Reference Key
zhang2019microrna152animals Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Zhang, Yong;Yan, Honglin;Zhou, Pan;Zhang, Zhenzhen;Liu, Jingbo;Zhang, Hongfu;
Journal animals
Year 2019
DOI
E669
URL
Keywords
meat quality ucp3 mir-152 myofiber specification myogenesis porcine myoblasts youth adolescents physical activity active video games gamification exergaming teens edge detection analog-to-digital converter cmos image sensor xor iris recognition

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