F Paramagnetic Relaxation-Based NMR for Quaternary Structural Restraints of Ion Channels.

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ID: 51450
2019
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Abstract
Quaternary distance restraints are essential to define the three-dimensional structures of protein assemblies. These distances often fall within a range of 10-18 Å, which challenges the high and low measurement limits of conventional nuclear magnetic resonance (NMR) and double electron-electron resonance electron spin resonance spectroscopies. Here, we report the use of F paramagnetic relaxation enhancement (PRE) NMR in combination with F/paramagnetic labeling to equivalent sites in different subunits of a protein complex in micelles to determine intersubunit distances. The feasibility of this strategy was evaluated on a pentameric ligand-gated ion channel, for which we found excellent agreement of the F PRE NMR results with previous structural information. The study suggests that F PRE NMR is a viable tool in extracting distance restraints to define quaternary structures.
Reference Key
bondarenko2019facs Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Bondarenko, Vasyl;Wells, Marta M;Chen, Qiang;Singewald, Kevin C;Saxena, Sunil;Xu, Yan;Tang, Pei;
Journal ACS chemical biology
Year 2019
DOI
10.1021/acschembio.9b00692
URL
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