Immunohistochemistry for Tumor-Infiltrating Immune Cells After Oncolytic Virotherapy.

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ID: 48804
2020
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Ranked #32 of 139 articles by views in methods in molecular biology (clifton, nj)

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Abstract
Immunohistochemistry (IHC) is an integral laboratory staining technique, which is used for the detection of immune cells in mouse/human tissues or tumors. Oncolytic herpes simplex virus (oHSV) treatment or virotherapy of solid tumors results in antitumor immune responses and infiltration of a variety of immune cells into the tumor. Here, we describe a step-by-step chromogen/substrate-based single- and dual-color IHC protocol to stain immune cells in formalin-fixed, paraffin-embedded mouse glioblastoma (GBM) brain tumor sections after oHSV virotherapy. Tumor sections are deparaffinized with xylene, then gradually rehydrated using ethanol, followed by heat-mediated antigen retrieval using appropriate buffers. Tumor sections are incubated with primary antibodies, which detect a specific immune cell antigen, then incubated with peroxidase- or phosphatase-labeled secondary antibodies, followed by incubation with a color-producing substrate and color visualization (of immune cells) by light microscopy. The protocol described herein is also applicable to detect immune cells in other mouse and human tumors or organs after other forms of immunotherapy.
Reference Key
saha2020immunohistochemistrymethods Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Saha, Dipongkor;Rabkin, Samuel D;
Journal methods in molecular biology (clifton, nj)
Year 2020
DOI
10.1007/978-1-4939-9794-7_11
URL
Keywords
immune cells immunohistochemistry antigen retrieval antigen–antibody reaction dual-color immunohistochemistry oncolytic herpes simplex virus substrate-based immunohistochemistry virotherapy

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