Metabolomic Profiling of Staphylococcus aureus.

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ID: 45628
2020
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Ranked #22 of 139 articles by views in methods in molecular biology (clifton, nj)

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Abstract
Metabolomics is becoming increasingly important in bioscience research as it provides a comprehensive analytical platform for a better understanding of the metabolic functions of cells and organisms. Recently, microbial metabolomics has been utilized in diverse research areas, including detection and diagnosis of pathogens, metabolic engineering, and drug discovery. An efficient and reproducible method to measure the intracellular metabolites of a specific microbial organism is a key prerequisite for utilizing metabolome analysis in microbiological research. In this chapter, we describe a workflow focusing on the extraction and quantification of intracellular metabolites of Staphylococcus aureus. Fast quenching with chilled methanol is applied to minimize metabolite leakage, while solvent extraction is used to obtain both polar and nonpolar fractions, which are then analyzed by respective liquid chromatography-mass spectrometry (LC-MS) methods for characterizing and quantifying the intracellular metabolites of S. aureus. This protocol is demonstrated to be an efficient method for analyzing polar and nonpolar intracellular metabolites of S. aureus.
Reference Key
lei2020metabolomicmethods Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Lei, Ting;Mao, Qingqing;Chen, Chi;Ji, Yinduo;
Journal methods in molecular biology (clifton, nj)
Year 2020
DOI
10.1007/978-1-4939-9849-4_13
URL
Keywords
staphylococcus aureus amino acids metabolome lc-ms mrsa

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