[The monitoring of laboratory indices of immune status of healthy children residing in conditions of intensive immunotropic zone of industrial town.]

The complex evaluation was implemented concerning to quantitative and functional indices of immune-competent cells of peripheral blood, cytokine and immunoglobulin profile of blood serum with detection of confidence ranges of immunologic parameters of healthy children aged 3-11 years (n=98) residing in intensive immunotropic zone with the highest ecological risk. The multi-parametric flow cytofluorimeter COULTER EPICS XL (Beckman Coulter Inc.) with selection of of panels of monoclonal antibodies with multi-color combination of fluorichromуes was applied to analyze leukocytes, sub-population composition of lymphocites and processes of activation of cells of peripheral blood. The levels of cytokines were detected using immunoenzyme technique applying reagents by «R&D Diagnostics Inc.» (USA). The reagents' kit of «Vektor-Best» (Novosibirsk) was applied to detect spontaneous and mitogen-induced production of cytokines by cells of total blood The immuneturbidimetry technique was applied to detect content of immunoglobulins in blood serum. The total and specific IgE was detected using solid-phase immune enzyme analysis with reagents' kits by «Alkor Bio Company» (St. Petersburg). The statistical data processing was implemented using software Statistica 10 with data verification 95%-99%. In junior school children as compared with children aged 3-6 years, statistically reliable greater number of leukocytes were detected with preservation of high ratio of neutrophils, prevalence of helper sub-population of T-lymphocytes, expressed activation of B-lymphocytes, higher levels of serum IgA, IgG, common and specific IgE (latent sensibilization by domestic allergens), prevalence of intensity of production of interleukins IL-13 over IL-4 and significantly higher provision with natural killers.