A new HPLC-MS/MS method for simultaneous determination of Cyclosporine A, Tacrolimus, Sirolimus and Everolimus for routine therapeutic drug monitoring.

Clicks: 433
ID: 40432
2019
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Abstract
A rapid, simple and robust HPLC-MS/MS method for simultaneous determination of immunosuppressants Cyclosporine A, Tacrolimus, Sirolimus and Everolimus has been developed and validated. Sample of whole blood with volume of 50 μL was prepared by a protein precipitation with methanol and 0.5 mol. L ZnSO. Chromatographic separation was achieved on a Phenyl-Hexyl column by a gradient elution using 20 mmol.L ammonium formate/0.1% (v/v) formic acid in water (mobile phase A) and 20 mmol.L ammonium formate/0.1% (v/v) formic acid in methanol (mobile phase B) with flow rate 1 mL.min. The run time was 3.5 min. Electrospray ionization and multiple reaction monitoring was used. The lower limit of quantification (LLOQ) was set at 0.5 μg.L for Tacrolimus, Sirolimus and Everolimus and 5 μg.L for Cyclosporine A. The method demonstrated adequate accuracy and precision with sufficient linearity range.
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krn2019ajournal Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Krnáč, Dušan;Reiffová, Katarína;Rolinski, Boris;
Journal journal of chromatography b, analytical technologies in the biomedical and life sciences
Year 2019
DOI
S1570-0232(19)30979-1
URL
Keywords Keywords not found

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