Cytosolic delivery of CRISPR/Cas9 ribonucleoproteins for genome editing using chitosan-coated red fluorescent protein.

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ID: 39312
2019
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Ranked #53 of 184 articles by views in Chemical communications (Cambridge, England)

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Abstract
Though plenty of viral and non-viral methods have been rapidly developed for the delivery of the CRISPR/Cas9 system, the direct delivery of Cas9 ribonucleoproteins (RNPs) into the nucleus for genome editing via the non-homologous end joining (NHEJ) or homology-directed repair (HDR) pathway, especially the latter one, remains a challenge. Here we report a delivery vehicle achieved by encapsulating red fluorescent protein (RFP) within chitosan (CS), which can simultaneously deliver engineered Cas9 RNPs with a poly-glutamate peptide tag (E-tag) and DNA donors into a range of cell types with high genome editing efficacy and non-cytotoxicity.
Reference Key
qiao2019cytosolicchemical Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Qiao, Jie;Sun, Wenli;Lin, Siyu;Jin, Rong;Ma, Lixin;Liu, Yi;
Journal Chemical communications (Cambridge, England)
Year 2019
DOI
10.1039/c9cc00010k
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