Assessment of PFAS toxicity using lux -marked bioluminescent Pseudomonas biosensors

Clicks: 4
ID: 338050
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #238 of 241 articles by views in FEMS microbiology letters

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 241 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Per- and polyfluoroalkyl substances (PFAS) are persistent fluorinated contaminants of growing environmental concern, yet rapid methods for assessing their biological effects remain limited. This study evaluated lux-marked Pseudomonas putida F1 Tn5 luxCDABE and P. putida 12708 Tn5 luxCDABE as whole-cell biosensors for detecting sublethal PFAS toxicity. Fluoride bioassays confirmed both strains tolerated up to 1,000 mg L-1 fluoride without measurable bioluminescence inhibition, supporting their suitability for PFAS exposure studies. Growth and bioluminescence responses were then assessed in M9 minimal medium following exposure to PFOA, PFNA, and PFHxS. Conventional growth measurements showed limited effects across tested concentrations, with strain-specific reductions observed mainly in P. putida F1 Tn5 luxCDABE. Bioluminescence revealed clearer compound- and concentration-dependent responses. PFOA and PFNA caused significant inhibition of peak bioluminescence in P. putida F1 Tn5 luxCDABE, while P. putida 12708 Tn5 luxCDABE showed a variable response, including increased luminescence following PFOA exposure and inhibition at higher concentrations of selected PFAS. These findings demonstrate that lux-marked P. putida biosensors are able to detect PFAS-induced metabolic stress with greater sensitivity than conventional growth assays and highlights their potential as rapid tools for ecotoxicological screening of fluorinated contaminants.
Reference Key
openalex_W7224861432 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Diana Senovilla‐Herrero, Katrina Campbell, Sarah Helyar, Heather Moore, Lenka Mbadugha
Are you one of these authors? Claim your profile
Journal FEMS microbiology letters
Year 2026
DOI
10.1093/femsle/fnag135
URL
https://academic.oup.com/femsle/advance-article-pdf/doi/10.1093/femsle/fnag135/72616532/fnag135.pdf
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.