Human in vitro airway models to assess point-of-contact respiratory toxicity of surfactants: development and evaluation of a repeated-exposure study design

Clicks: 3
ID: 338046
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #152 of 152 articles by views in toxicological sciences : an official journal of the society of toxicology

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 152 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Repeated-dose exposure studies can provide insights into the toxicity of test substances, including dose-response relationships, cumulative effects, delayed responses, and reversibility of effects. A limited number of in vitro studies have assessed the effects of repeated exposure of respiratory cells to chemicals applied as liquids (via direct pipetting). The work presented in this paper builds upon these published in vitro repeated-dose studies to develop and evaluate a repeat-exposure study design for use with reconstructed human respiratory epithelium (RHRE). To this end, a bronchial RHRE (MucilAir, Epithelix) was exposed to 30 µL (91 µL/cm²) of two surfactants-oleoyl sarcosine and Triton X-100-for 4 hours on up to 4 consecutive days. The results showed that the repeat-exposure protocol can be used to detect toxic effects, including differences in the responses caused by each surfactant. More specifically, while the effects of oleoyl sarcosine exposure increased over 4 days, the effects of Triton X-100 were the strongest on day 1 and decreased over the course of the experiment. This work helped refine a repeated-dose protocol that can be adapted to assess other chemistries when the effects of repeated exposure are of interest, and underscores the importance of key parameters, such as endpoint selection, timepoints, exposure duration, and washing schedules.
Reference Key
openalex_W7224842853 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Monita Sharma, A. Stucki, Sandra Verstraelen, Karen Hollanders, An Jacobs, Nuria Roldán, Sylvie Rémy, Adam Bettman, Amy J. Clippinger
Journal toxicological sciences : an official journal of the society of toxicology
Year 2026
DOI
10.1093/toxsci/kfag146
URL
https://academic.oup.com/toxsci/advance-article-pdf/doi/10.1093/toxsci/kfag146/72616275/kfag146.pdf
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.